Résumé

P212

LC-MS/MS identification and beneficial effects of Phoenix dactylifera L. leaves hydroalcoholic extract: in vitro and in vivo studies of glycosidase inhibitors

Dr B. KHEMAKHEM^a, Mlle M. CHAKROUN^a, Mlle H. EL ABED^a, M. M. MAKNI^b, Pr M. BOUAZIZ^c, Pr N. DRIRA^a, Pr H. MEJDOUB^a

^a Laboratory of Plant Biotechnology, Sfax Faculty of Sciences. Tunisia, sfax ; ^b Laboratory of Toxicology, Environmental Microbiology and Health, Sfax Faculty of Sciences. Tunisia, Sfax ; ^c Electro-Chemical Environmental Laboratory, ENIS, Sfax.Tunisia, Sfax

Phoenix dactylifera L. has an important role in the Tunisian social, economic, and ecological sectors. LC–MS/MS analysis of leaves hydroalcoholic extract from Phoenix dactylifera L. showed the presence of flavonoid glycosoides. The in vitro study showed that the extract exhibited a more specific and potent inhibitor of α-glucosidase with an IC₅₀ value (20 ±1 µg/mL) than α-amylase (30 ±0.8 µg/mL). The in vivo study of the postprandial hyperglycemia activity with this extract in the gastrointestinal absorption loaded with an overdose of maltose showed a decrease in plasma glucose levels after 60 min in resemblance to the acarbose effect. These results confirmed the anti-hyperglycemic effect of the hydroalcoholic extract of Phoenix dactylifera L. leaves, which could lend support for its pharmaceutical use. 

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